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Service List: Molecular biology and omics

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Access to DNA sequencing

University of Vigo

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Instrument: Sequencer Brand/Type: Sanger AB3130 sequencer
Instrument: Sequencer Brand/Type: NGS Ion Torrent Proton
Instrument: Sequencer Brand/Type: NGS Ion Torrent PGM

Access to archiving of turbellarians

University of Hasselt

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Instrument: QPCR Brand/Type: Applied Biosystems 7900 RT-PCR
Instrument: QPCR Brand/Type: Applied Biosystems 7500 RT-PCR

Access to molecular biology facilities

Oceanographic Observatory of Banyuls sur mer

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Instrument: Electrophoresis 1D Brand/Type: Ettan IPGPhor 3
Instrument: Microplate spectrometer Brand/Type: Paradigm
Instrument: Ultracentrifuge Brand/Type: Optima Max XP
Instrument: Laser scanner for quantification of proteins, nucleic acids and other biomolecules Brand/Type: Typhoon FLA9000
Instrument: Electrophoresis 2D Brand/Type: Ettan Dalt Six
Instrument: Semi-dry blotter Brand/Type: SemiPhor semi-dry transfer unit TE77_PWR
Instrument: Stitching software and sequence alignment Brand/Type: CodonCode Aligner
Instrument: Primer 6 software
Instrument: GeneMapper software
Instrument: Automated workstation Brand/Type: Biomek 3000
Instrument: Spectrophotometer (1-2 ?l volumes) Brand/Type: Nanovue
Instrument: DNA/RNA/protein extractor Brand/Type: Maxwell 16 Mdx
Instrument: PCR machine Brand/Type: GeneAmp ABI 2700
Instrument: PCR machines Brand/Type: 4 Veriti ABI (0.1-0.2ml)
Instrument: PCR machine in real time (qPCR) Brand/Type: StepOne Plus ABI
Instrument: Bioanalyzer DNA, RNA, Proteins, cells Brand/Type: Agilent 2100
Instrument: Microcentrifuges Brand/Type: 2 Micro star 17
Instrument: Refrigerated centrifuge (microplate, microtube, falcon) Brand/Type: Heraeus Multifuge 3S-R
Instrument: Heated microtube shaker (1.5-2 ml) Brand/Type: 2 Thermomixers (Eppendorf, Grant-Bio)
Instrument: Mill Mixer Brand/Type: Retsch MM200
Instrument: Refrigerated centrifuge (microtube, falcon) Brand/Type: Sigma 2-16KC
Instrument: Genetic Analyzer (DNA sequencing, fragment analysis) Brand/Type: 3130XL ABI

Access to basic molecular biological analysis

Istituto di scienze marine Venice

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Instrument: Thermal cycler Brand/Type: xxx
Instrument: Laminar flow hood Brand/Type: xxx
Instrument: Incubators
Instrument: DNA quantification Brand/Type: Life Technology Qubit
Instrument: Computing facility for bioinformatics
Instrument: High resolution DNA fragment analysis Brand/Type: Qiagen Qiaxel
Instrument: PCR cyclers Brand/Type: Eppendorf T-Gradient
Instrument: QPCR Brand/Type: Qiagen Rotorgene Q

Access to DNA barcoding of fish and larvae

Interuniversity Institute for Marine Sciences

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Instrument: Rt-PCR
Instrument: Nanodrop
Instrument: PCR

Access to basic molecular biological analysis

National Institute of Oceanography and Experimental Geophysics

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Instrument: Thermocycler Brand/Type: Eppendorf Master cycler gradient

Access to marine bacterial platform

Ghent University

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Instrument: Biosafety cabinet suitable for hazardous chemicals Brand/Type: Nuaire LabGard NU-440-600E
Instrument: Liquid handler Brand/Type: Viaflo 96 ? INTEGRA Biosciences
Instrument: MALDI-TOF Mass Spec Brand/Type: Applied Biosystems/MDS SCIEX 4800 MALDI TOF/TOF
Instrument: Robotic colony picker Brand/Type: Kbiosystems K2

Access to marine molecular platform

Ghent University

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Instrument: DNA extraction lab for non-toxic materials
Instrument: DNA extraction lab for toxic materials (biosafety class 2 type A2)
Instrument: Temperature Control Room
Instrument: 2 PCRs (+UV working station)
Instrument: QPCR
Instrument: Quantification and quality control of DNA, RNA and protein Brand/Type: Qubit fluorometer
Instrument: Quantification and quality control of DNA, RNA and protein Brand/Type: Agilent 2100 Bioanalyzer
Instrument: Spectrophotometer Brand/Type: Nanodrop
Instrument: DGGE and staining Brand/Type: xxx

Access to molecular biological analysis of marine organisms

Plentzia Marine Station, University of the Basque Country

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Instrument: Centrifuges
Instrument: Ultracentrifuges
Instrument: Bidimensional gel electrophoresis/proteomics Brand/Type: PROTEAN
Instrument: Multimode microplate reader imaging Brand/Type: BioTek Citation 5
Instrument: Biophotometer Brand/Type: Eppendorf
Instrument: Room Temp DNA storing archive Brand/Type: GenVault
Instrument: Controlled freezing device Brand/Type: SY LAB, IceCube 14S-B
Instrument: Cryogenic deposit Brand/Type: ESPACE 331
Instrument: Liquid Nitrogen automatic filler Brand/Type: Air Liquide
Instrument: Cryogenic reservoirs Brand/Type: cryotherm chronos
Instrument: Ultrafreezers Brand/Type: Thermo
Instrument: Miscroscopy slide storage Brand/Type: Bio Optica
Instrument: Fluorimeter/luminometer Brand/Type: BioTek FLx800 TBIE
Instrument: Spectrophotometer microplates Brand/Type: BioTek 6709 Epoch
Instrument: Thermocylcer Brand/Type: PTC-200 MJ Research
Instrument: Pipetting Robot Brand/Type: Tecan Freedom EVO
Instrument: Thermocylcer Brand/Type: PTC-100 MJ Research
Instrument: Thermocycler Brand/Type: Surecycler Agilent Tech
Instrument: QPCR thermocycler Brand/Type: ABI7300
Instrument: Bioanalyzer 2100 Brand/Type: Agilent Technologies Bioanalyzer 2100
Instrument: Gel documentation System Brand/Type: Syngene G:Box
Instrument: DNA/RNA electrophoresis Brand/Type: xxx
Instrument: Spectrophotometer Brand/Type: BioTek Elx-808
Instrument: Incubation chambers Brand/Type: xxx
Instrument: Autoclaves Brand/Type: xxx
Instrument: Tissue homgenizer Brand/Type: Precellys 24, Bertin
Instrument: QPCR thermocycler Brand/Type: ViiA7 Life Technologies (364 weel plates)

Access to proteomics facilities

University of Vigo

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Instrument: Gel based proteomics Brand/Type: Chemidoc FRX
Instrument: Gel based proteomics Brand/Type: Pharos FX+
Instrument: Gel based proteomics Brand/Type: Proteineer DP & SP
Instrument: Gel based proteomics Brand/Type: MALDI TOF AFIII
Instrument: HPLC based proteomics Brand/Type: FTMS Apex IV
Instrument: HPLC based proteomics Brand/Type: Akta Purifier
Instrument: HPLC based proteomics Brand/Type: Orbitrap

Access to molecular biology facilities

Stazione Zoologica Anton Dohrn of Naples

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Instrument: Semiconductor Sequencer Brand/Type: Life Technologies Ion Proton
Instrument: Robotic Liquid Handler Brand/Type: Tecan 200 Freedom Eva dual arms
Instrument: DNA Analyzer
Instrument: DNA Analyzer Brand/Type: ABI 3730 48 capillaries
Instrument: QPCR Brand/Type: Life Technologies ViiA7 384 well plates
Instrument: DdPCR Brand/Type: BioRad QX200 Droplet Reader
Instrument: Microfluidic chip analyzer Brand/Type: Agilent 2100 Bioanalyzer

Access to next generation sequencing

Station biologique de Roscoff

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Instrument: NGS sequencer Brand/Type: Illumina MiSeq

Access to genomics facilities

Interdisciplinary Centre of Marine and Environmental Research

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Instrument: RtPCR supporting equipment
Instrument: RtPCR Brand/Type: xxx
Instrument: RtPCR Brand/Type: xxx
Instrument: RtPCR Brand/Type: xxx

Access to molecular biology facilities

Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research

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Instrument: Fluorometric quantitation Brand/Type: Qubit Fluorometric quantitation
Instrument: Spectrophotometer Brand/Type: xxx
Instrument: Fluorometer with UV/Blue Channel Brand/Type: Quantifluor-ST Fluorometer
Instrument: DNA large fragment shearing Brand/Type: Digilab Hydroshear
Instrument: Gel Electrophoresis System Brand/Type: xxx
Instrument: Gel Electrophoresis System Brand/Type: BioRAD
Instrument: Tank Control System
Instrument: Spectrophotometer Brand/Type: Nanodrop 1000
Instrument: Electroelution system Brand/Type: Whatman Elutrap
Instrument: Magnetic racks Brand/Type: DNA purification, size selection
Instrument: DNA shearing Brand/Type: Compressed Nitrogen gas
Instrument: Disperser wokstation Brand/Type: IKA Ultra-Turrax
Instrument: Sizing, quantitation and quality control of DNA, RNA, proteins Brand/Type: Agilent 2100?BioAnalyzer
Instrument: Gel documentation, blue/UV light Brand/Type: Nippon Gel Documentation system
Instrument: Electroporator Brand/Type: xxx
Instrument: Genome Sequencer Brand/Type: 454 Roche FLX
Instrument: Genome Sequencer Brand/Type: 454 GS Junior
Instrument: Genome Sequencer Brand/Type: Illumina MiSeq
Instrument: Robotic sample manipulator Brand/Type: Beckman Biomek 2000
Instrument: Automatic Sequencer Brand/Type: ABI 3730
Instrument: Device for automatic DNA & RNA extraction Brand/Type: Qiacube
Instrument: Open Array Real Time PLR System Brand/Type: Applied Biosystems
Instrument: RealTime Thermocycler Brand/Type: MJ Opticon 2
Instrument: Tissue Lyser Brand/Type: QIAGEN TissueLyser II
Instrument: Coulter Counter Brand/Type: Beckman Coulter
Instrument: Biological Safety Cabinet Brand/Type: Telstar BIO II A Class II
Instrument: Thermal cyclers Brand/Type: BioRAD
Instrument: RealTime Thermocycler Brand/Type: Roche LightCycler 1.5
Instrument: Array Scanner

Access to molecular biology services

Institute for coastal marine environment Capo Granitola

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Instrument: Semiconductor Sequencer Brand/Type: ION PROTON (Life Technologies)
Instrument: Robotic liquid handler Brand/Type: 200 Freedom Evo (Tecan) dual arms
Instrument: DNA Analyzer Brand/Type: 3730 (ABI) 48 capillaries
Instrument: DdPCR Brand/Type: QX200 BioRad Droplet Reader
Instrument: Microfluidic chip analyzer Brand/Type: 2100 Bioanalyzer (Agilent)
Instrument: QPCR Brand/Type: ViiA7( Life Technologies)384 well plates
Instrument: RT-PCR Brand/Type: 7500 HT Fast Real-Time PCR System (Thermo Fisher)

Access to molecular biology facilities

Centre of Marine Sciences

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Instrument: QPCR Brand/Type: Applied Biosystems StepOnePlus
Instrument: DGGE
Instrument: QPCR Brand/Type: BIORAD iCycler
Instrument: Sequencer Brand/Type: Applied Biosystems genetic Analyzer 3130xl
Instrument: Tissue Homogeneizer Brand/Type: Bertin, Precellys 24
Instrument: Nano volume spectrophotometer Brand/Type: Anaytikjena, ScanDrop 200

Access to molecular biology facilities

Scottish Association for Marine Science

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Instrument: Rt-qPCR Brand/Type: ???
Instrument: PCR Brand/Type: ???

Access to marine bacterial platform

Institute for coastal marine environment Messina

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Instrument: Fume Hood
Instrument: Balance
Instrument: Extraction system Brand/Type: Buchi Soxhlet 4 channels B-811
Instrument: Stirring hotplate Brand/Type: Thermo stirring
Instrument: Ultrasonic tank
Instrument: GC-FID Brand/Type: Dani Master GC with autosampler, injectors PTV and SSL, FID e TC
Instrument: Evaporator Brand/Type: Reactitherm
Instrument: Optical microscope Brand/Type: Zeiss Axioplan2
Instrument: Photometer Brand/Type: Eppendorf
Instrument: Rt-PCR Brand/Type: Applied Biosystem 7300
Instrument: Thermocycler Brand/Type: BioRAD
Instrument: Incubator Brand/Type: Certomat
Instrument: Spectrophotometer Brand/Type: CELBIO Nanodrop ND-1000
Instrument: Centrifuge Brand/Type: Eppendorf
Instrument: Image analyser Brand/Type: BioRAD
Instrument: Electrophoresis Brand/Type: BioRAD
Instrument: Incubator Brand/Type: Pselecta

Access to environmental DNA laboratory and facilities

Flanders Marine Institute

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